Investigation on the precipitation, microenvironment and recovery of protein in CO2-expanded reverse micellar solution

A New Thermodynamic Approach for Protein Partitioning in Reverse Micellar Solution

Reverse micellar systems are nanofluids with unique properties that make them attractive in high selectivity separation processes, especially for biological compounds. Understanding the phase behavior and thermodynamic properties of these nanosystems is the first step in process design. Separation of components by these nanosystems is performed upon contact of aqueous and reverse micellar phase...

Fluorescence studies on the microenvironments of proteins in CO2-expanded reverse micellar solutions

The effect of compressed CO2 on the microenvironment of the two proteins (cytochrome c and RNase A) in sodium bis(2-ethylhexyl)sulfosuccinate (AOT) reverse micelles was investigated using high-pressure steady-state fluorescence and time-resolved fluorescence. It is found that RNase A can shift from the water core to the interface of the reverse micellar cores as CO2 is dissolved, while cytochro...

A Simple Complexation Model and the Experimental Datafor Protein Extraction Using Reverse Micellar Systems

Effect of Compressed Co2 on the Properties of Aot in Isooctane Reverse Micellar Solution and Its Application to Recover Nanoparticles

Effect of compressed CO2 on properties of the sodium bis(2-ethylhexyl) sulfosuccinate (AOT)/isooctane/water reverse micellar solution has been studied by phase behavior measurement. UV-vis study indicates that TiO2 in the reverse micelles can be precipitated by compressed CO2 at suitable pressures, while the surfactant AOT remains in the solution. Using this concept, TiO2 nanoparticles synthesi...

Influence of CO2 Dissolution and Asphaltene Precipitation on Oil PVT and Recovery